Open AccessVirgibacillus phasianinus sp. nov., a halophilic bacterium isolated from faeces of a Swinhoe's pheasant, Lophura swinhoii
Author(s) -
Euon Jung Tak,
Hyun Sik Kim,
June-Young Lee,
Woorim Kang,
Hojun Sung,
Pil Soo Kim,
Dong-Wook Hyun,
Na-Ri Shin,
Jeong Rae Roh,
Sun Duk Park,
Hyung Eun Shim,
Jin-Woo Bae
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002650
Subject(s) - biology , 16s ribosomal rna , diamino acid , microbiology and biotechnology , strain (injury) , phylogenetic tree , fatty acid , bacteria , gene , biochemistry , genetics , gene sequence , anatomy
A rod-shaped, Gram-stain-positive, motile and aerobic bacterium, designated LM2416 T , was isolated from faeces of Lophuras winhoii living in Seoul Grand Park, Gyeonggi-do, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain LM2416 T belonged to the genus Virgibacillus, sharing high 16S rRNA gene sequence similarities to Virgibacillus necropolis LMG 19488 T (99.0 %), Virgibacillus carmonensis LMG 20964 T (98.4 %), Virgibacillus arcticus Hal 1 T (98.3 %) and Virgibacillus flavescens S1-20 T (97.9 %). The isolate grew at 10-30 °C, pH 6-7 and 0-20 % (w/v) NaCl. Optimal growth was observed at 30 °C, pH 6-7 and 10 % (w/v) NaCl. The major fatty acid was anteiso-C15 : 0. Polar lipids were composed of phosphatidylglycerol, diphosphatidylglycerol, three unknown phospholipids and two unknown aminophospholipids. The main menaquinone was MK-7. Strain LM2416 T had alanine, lysine, glutamic acid, glycine and aspartic acid as cell-wall amino acids and ribose as a cell-wall sugar. The whole genome sequences of strain LM2416 T and V. necropolis KCTC 3820 T were sequenced by PacBio RS II sequencing. The genome sequence-based G+C content of strain LM2416 T was 39.5 mol%. The orthologous average nucleotide identity value, showing genetic relatedness between strain LM2416 T and V. necropolis KCTC 3820 T , was 78.3 %. Based on the phylogenetic, biochemical, chemotaxonomic and genotypic data presented in this study, strain LM2416 T is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus phasianinus is proposed. The type strain is LM2416 T (=KCTC 33927 T =JCM 32144 T ).