Open AccessSaturnispora kantuleensis f.a., sp. nov., a novel yeast species isolated from peat in a tropical peat swamp forest in Thailand
Author(s) -
Pannida Khunnamwong,
Savitree Limtong
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002641
Subject(s) - biology , internal transcribed spacer , swamp , phylogenetic tree , peat , ribosomal rna , botany , genus , yeast , taxonomy (biology) , gene , ecology , genetics
Two strains (DMKU-PPS4-5 T and DMKU-EPS3-4) representing a single novel anamorphic yeast species were isolated from two peat samples collected in Kan Tulee peat swamp forest, Surat Thani Province, Thailand. The strains differed by two nucleotide substitutions in the sequences of the D1/D2 region of the large subunit (LSU) rRNA gene and only one nucleotide substitution in the internal transcribed spacer (ITS) region. Phylogenetic analysis based on the D1/D2 regions showed that the two strains represented a single species in the genus Saturnispora and were clearly distinct from other related species. Saturnispora sekii was the most closely related species, but with 1.7-2.1 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene, and 3.1-3.3 % nucleotide substitutions in the ITS region. They therefore represent a novel species of the genus Saturnispora, although the formation of ascospores was not observed. The name Saturnispora kantuleensis f.a., sp. nov. is proposed. The type strain is DMKU-PPS4-5 T (=CBS 15217 T =TBRC 7762 T ).