Open AccessGeorgenia deserti sp. nov., a halotolerant actinobacterium isolated from a desert sample
Author(s) -
Wael N. Hozzein,
Ziwen Yang,
Sulaiman Ali Alharbi,
Waleed A. A. Alsakkaf,
Mipeshwaree Devi Asem,
Min Xiao,
Nimaichand Salam,
Wenjun Li
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002640
Subject(s) - biology , 16s ribosomal rna , halotolerance , strain (injury) , rhamnose , microbiology and biotechnology , phylogenetic tree , botany , gene , galactose , bacteria , biochemistry , genetics , anatomy
Strain SYSU D8004 T was isolated from a sample collected from an arid area in Saudi Arabia. The isolate was Gram-stain-positive, non-motile, aerobic and non-spore-forming. It could grow at 4-45 °C, at pH 6.0-10.0 and in the presence of up to 17 % (w/v) NaCl. Pairwise comparison of the 16S rRNA gene sequences indicated that strain SYSU D8004 T shared highest sequence similarity with Georgenia halophila YIM 93316 T (96.5 %). Menaquinone MK-8(H4) was detected as the respiratory quinone. The polar lipid profile of strain SYSU D8004 T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylinositol mannosides, two unidentified phospholipids and an unidentified glycolipid. Strain SYSU D8004 T contained anteiso-C15 : 0, iso-C15 : 0 and C14 : 0 as the predominant fatty acids (>10 %). Galactose, glucose and rhamnose were detected as whole-cell sugars. Based on analyses of the phenotypic, genotypic and phylogenetic characteristics, it was determined that strain SYSU D8004 T could be differentiated from other closely related members of the genus Georgenia. Strain SYSU D8004 T is therefore considered to represent a novel species of the genus Georgenia, for which the name Georgenia deserti sp. nov. is proposed. The type strain is SYSU D8004 T (=CGMCC 1.15793 T =KCTC 39987 T ).