Open AccessPaenibacillus translucens sp. nov., isolated from tidal flat sediment
Author(s) -
Yong-Seok Kim,
Chang-Jun Cha
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002613
Subject(s) - 16s ribosomal rna , biology , peptidoglycan , strain (injury) , microbiology and biotechnology , paenibacillus , phylogenetic tree , bacteria , ribosomal rna , glycolipid , diamino acid , terpenoid , diaminopimelic acid , gene , botany , gene sequence , biochemistry , genetics , anatomy
A Gram-stain-variable, aerobic, rod-shaped, motile and spore-forming bacterial strain, designated CJ11 T , was isolated from a tidal flat sediment sample from Ganghwa-do, Republic of Korea. Strain CJ11 T grew optimally on R2A at 30 °C and pH 7.0. Sequencing results of the 16S rRNA gene revealed that strain CJ11 T possesses two copies of the 16S rRNA gene varying at five nucleotide positions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ11 T belonged to the genus Paenibacillus within the family Paenibacillaceae and was most closely related to Paenibacillus lacus KCTC 33691 T (99.36-99.15 % similarity). DNA-DNA relatedness levels of strain CJ11 T was 41.7 % (reciprocal, 57.8 %) to P. lacus KCTC 33691 T . The G+C content of the genomic DNA was 51.0 mol%. Strain CJ11 T contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The major isoprenoid quinone was menaquinone-7. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified glycolipid and several unidentified lipids. On the basis of the polyphasic taxonomic study, strain CJ11 T represents a novel species in the genus Paenibacillus, for which the name Paenibacillustranslucens sp. nov. is proposed. The type strain is CJ11 T (=KACC 19304 T =JCM 32080 T ).