Open AccessProposal of Litorimonas haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona
Author(s) -
Johannes Schellenberg,
HansJürgen Busse,
Martin Hardt,
Patrick Schubert,
Thomas Wilke,
Peter Kämpfer,
Stefanie P. Glaeser
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002592
Subject(s) - biology , diamino acid , 16s ribosomal rna , peptidoglycan , spermidine , strain (injury) , sponge , brevibacterium , microbiology and biotechnology , genomic dna , glycolipid , diaminopimelic acid , bacteria , botany , biochemistry , dna , cell wall , genetics , gene sequence , anatomy , microorganism , enzyme
A bright-orange-pigmented, Gram-stain-negative, motile, and rod-shaped bacterium, strain MAA42 T , was isolated from a marine sponge of the genus Haliclona, which is in long-time culture in a marine aquarium system at the Justus Liebig University Giessen, Germany. The strain grew at 4-34 °C (optimum 28 °C), in the presence of 0.5-9.5 % (w/v) NaCl (optimum 3.5 %) and at pH 4.5-10.0 (optimum pH 7.5). Strain MAA42 T shared the highest 16S rRNA gene sequence similarity (98.1 %) with the type strain of Litorimonas taeanensis. Sequence similarities to all other closely related type strains were below 97 %. DNA-DNA hybridization of strain MAA42 T with L. taeanensis DSM 22008 T resulted in values of 4.7 % (reciprocal 17.7 %). Major cellular fatty acids of strain MAA42 T were C18 : 1ω7c (66.2 %), C18 : 1 2-OH (17.4 %), and C18 : 0 (14.1 %). Spermidine was predominant in the polyamine pattern, and ubiquinone Q-10 was the major respiratory quinone. The polar lipid profile contained the major compounds phosphatidylglycerol, monoglycosyldiglyceride, three unidentified phospholipids, and one unidentified glycolipid. Glucuronopyranosyldiglyceride was present as a minor compound. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The genomic DNA G+C content was 52.8 mol%. Based on the genotypic, chemotaxonomic, and phenotypic analyses, strain MAA42 T represents a novel species of the genus Litorimonas, for which the name Litorimonas haliclonae is proposed. The type strain is MAA42 T (=CCM 8709 T =CIP 111178 T =LMG 29765 T ).