Open AccessRoseomonas fluminis sp. nov. isolated from sediment of a shallow stream
Author(s) -
Yongseok Ko,
Jeeyeo Yim,
Woon Mo Hwang,
Keunsoo Kang,
TaeYoung Ahn
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002578
Subject(s) - biology , sediment , paleontology
An aerobic, Gram-negative, motile by means of a single polar flagellum, and ovoid-shaped bacterium, designated D3 T , was isolated from shallow stream sediments in Sinan-gun, South Korea. Growth occurred at 15-40 °C (optimum 35 °C), at pH 7.0-8.0 (optimum pH 7.0), and at an optimum NaCl concentration of 0.5 % (w/v). The major cellular fatty acids (>7 % of the total) were C16 : 0, C18 : 0 2-OH, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The predominant quinone was ubiquinone-10, and the G+C content of the genomic DNA of strain D3 T was 73.1 mol%. The major polyamine was spermidine. The major polar lipids of the isolate were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D3 T clustered with Roseomonas aquatica TR53 T within the genus Roseomonas. The 16S rRNA gene sequence of strain D3 T showed the highest sequence similarity to R. aquatica TR53 T (95.9 %), followed by Roseomonas rosea 173-96 T (95.7 %) and Roseomonas aerilata 5420S-30 T (95.0 %). Based on the phenotypic, phylogenetic and chemotaxonomic characterization, strain D3 T represents a novel species of the genus Roseomonas, for which the name Roseomonas fluminis sp. nov. is proposed. The type strain is D3 T (=KACC 19269 T =JCM 31968 T ).