Open AccessLactococcus reticulitermitis sp. nov., isolated from the gut of the subterranean termite Reticulitermes speratus
Author(s) -
Masahiro Yuki,
Mitsuo Sakamoto,
Yuki Nishimura,
Moriya Ohkuma
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002549
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , lactococcus , strain (injury) , hindgut , phylogenetic tree , coccus (insect) , ribosomal rna , bacteria , lactococcus lactis , gene , genetics , botany , lactic acid , larva , anatomy , midgut
Strain Rs-Y01 T was isolated from the gut of the wood-feeding subterranean termite Reticulitermes speratus. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain Rs-Y01 T belonged to the genus Lactococcus and was most closely related to Lactococcus raffinolactis JCM 5706 T with 98.1 % similarity in the 16S rRNA gene, followed by Lactococcus piscium JCM 16647 T (97.2 %). Genomic comparisons of strain Rs-Y01 T with L. raffinolactis JCM 5706 T were made using the Genome-to-Genome Distance Calculator and average nucleotide identity analysis (values indicated 29.2 and 84.6 %, respectively). Strain Rs-Y01 T was a Gram-stain-positive, facultatively anaerobic, non-motile coco-bacilli and formed l-lactic acid. The sugar fermentation and enzyme reactions of strain Rs-Y01 T differed from those of other species of the genus Lactococcus. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c/C18 : 1ω6c; 32.0 %), C16 : 0 (29.7 %) and C14 : 0 (18.1 %). Based on these characteristics, strain Rs-Y01 T represents a novel species of the genus Lactococcus, for which the name Lactococcusreticulitermitis sp. nov. is proposed. The type strain is Rs-Y01 T (=JCM 32106 T =DSM 105715 T ).