Open AccessHymenobacter defluvii sp. nov., isolated from wastewater of an acidic water neutralization facility
Author(s) -
Joo Won Kang,
Seon Choi,
Han Choe,
Chi Nam Seong
Publication year - 2018
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002497
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , microbiology and biotechnology , lineage (genetic) , bacteria , gene , biochemistry , genetics , anatomy
A non-motile, pink-coloured and rod-shaped bacterium, designated strain POA9 T , was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-reaction-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were C16 : 1ω5c, iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and anteiso-C15 : 0. The strain contained MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The DNA G+C content was 57 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain POA9 T forms a distinct evolutionary lineage within the radiation enclosing the members of the genus Hymenobacter, sharing the highest similarity with Hymenobacter actinosclerus CCUG 39621 T (95.5 % sequence similarity) followed by Hymenobacterseoulensis 16F7G T (95.5 %), Hymenobactertibetensis XTM003 T (95.4 %), Hymenobacterrutilus K2-33028 T (95.4 %) and Hymenobacter psychrotolerans Tibet-IIU11 T (94.9 %). A number of phenotypic characteristics distinguished strain POA9 T from the related members of the genus Hymenobacter. On the basis of the evidence presented in this study, a novel species, Hymenobacter defluvii sp. nov., is proposed for strain POA9 T (=KCTC 52270 T =JCM 31658 T ).