Open AccessKineococcus terrestris sp. nov. and Kineococcus aureolus sp. nov., isolated from saline sediment
Author(s) -
Fei Xu,
Qinyuan Li,
Gui-Ding Li,
Xiu Chen,
Yi Jiang,
ChengLin Jiang
Publication year - 2017
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002380
Subject(s) - biology , microbiology and biotechnology , 16s ribosomal rna , coccus (insect) , diamino acid , actinobacteria , strain (injury) , bacteria , botany , genetics , gene sequence , anatomy
Two novel actinobacteria, designated YIM 121936 T and YIM 121940 T , were isolated from alkaline sediment in Yuanjiang, China. The cells of the novel strains were Gram-stain-positive, aerobic, motile, non-spore-forming and coccus-shaped. The two strains both contained meso-diaminopimelic acid as the diagnostic diamino acid. The whole-cell sugars were arabinose, galactose, glucose, mannose and ribose. The predominant menaquinone was MK-9(H2). The polar lipid profile of both strains comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unknown phosphoglycolipid and five unknown phospholipids. The predominant fatty acids of both strains were anteiso-C15 : 0. The genomic DNA G+C contents of strains YIM 121936 T and YIM 121940 T were 74.7 and 75.2 %, respectively. Strain YIM 121936 T was closely related to Kineococcus aurantiacus IFO 15268 T (97.19 %), Kineococcus gypseus YIM 121300 T (97.00 %) and Kineococcus mangrovi NBRC 110933 T (97.00 %). Strain YIM 121940 T was closely related to K. aurantiacus IFO 15268 T (97.41 %), Kineococcus endophytica KLBMP 1274 T (97.18 %), Kineococcus rhizosphaerae RP-B16 T (97.09 %), Kineococcus radiotolerans SRS 30216 T (97.09 %), K. gypseus YIM 121300 T (97.00 %) and K. mangrovi NBRC 110933 T (97.00 %). Strain YIM 121936 T shared high 16S rRNA gene sequence similarity (99 %) with YIM 121940 T . Similarities of two strains with other species of the genus Kineococcus were <97 %. The DNA-DNA hybridization values were below 70 % among all the strains studied. YIM 121936 T and YIM 121940 T are representatives of two new species in the genus Kineococcus, for which names Kineococcus terreus sp. nov. (type strain YIM 121936 T =KCTC 39738 T =DSM 102155 T ) and Kineococcus aureolus sp. nov. (type strain YIM 121940 T =KCTC 39739 T =DSM 102158 T ) are proposed, respectively.