Open AccessSphingobacterium corticis sp. nov., isolated from bark of Populus × euramericana
Author(s) -
Yong Li,
Guantang Xu,
Ju-pu Chang,
Limin Guo,
Xiutao Yang,
Chungen Piao
Publication year - 2017
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.002210
Subject(s) - biology , 16s ribosomal rna , strain (injury) , bacteria , oxidase test , bark (sound) , microbiology and biotechnology , botany , biochemistry , enzyme , genetics , ecology , anatomy
A Gram-stain negative, aerobic, non-motile bacterial strain, 23D10-4-9 T , was isolated from symptomatic canker bark tissue of Populus × euramericana. The isolate grew between 4 and 35 °C, with optimal growth occurring at 25 °C. The species was positive for catalase and negative for oxidase activity. Nitrate was not reduced to nitrite. It showed activities toward β-galactosidase and β-glucosidase. Citrate was not utilized. Acid was produced from d-glucose. The major fatty acids were iso-C15 : 0, C16 : 1ω7c and iso-C17 : 0 3-OH. The main polar lipid profiles of the novel isolate included phosphatidylethanolamine, phospholipids and seven unknown lipids. The predominant menaquinone of the novel isolate was MK-7. The DNA G+C content was 40.6 mol%. 16S rRNA gene data revealed that the novel isolate shares the greatest sequence similarity with Sphingobacterium populi 7Y-4 T (96.1 %). Based on phenotypic and genotypic characteristics, the isolate represents a novel species within the genus Sphingobacterium, for which the name Sphingobacteriumcorticis sp. nov. is proposed. The type strain is 23D10-4-9 T (=CFCC 12640 T =KCTC 42248 T ).