Open AccessHymenobacter tenuis sp. nov., isolated from wastewater of an acidic water neutralization facility
Author(s) -
Joo Won Kang,
Ji Hee Lee,
Han Choe,
Chi Nam Seong
Publication year - 2017
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.001918
Subject(s) - biology , neutralization , wastewater , microbiology and biotechnology , virology , virus , waste management , engineering
A non-motile, red-pink-coloured, rod-shaped bacterium, designated strain POB6T, was isolated from a wastewater treatment facility, Republic of Korea. Cells were Gram-stain-negative, aerobic, catalase-positive and oxidase-negative. The major fatty acids were iso-C15 : 0, C16 : 1ω5c, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The strain contained menaquinone MK-7 as the only isoprenoid quinone, phosphatidylethanolamine as the major polar lipid and sym-homospermidine as the major polyamine. The DNA G+C content was 61 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain POB6T forms a distinct evolutionary lineage within the radiation enclosing the members of the genus Hymenobacter, sharing the highest similarity with Hymenobacterrigui WPCP131T (97.5 %) followed by Hymenobacter xinjiangensis X2-1gT (97.2 %), Hymenobacter perfusus A1-12T (97.2 %) and Hymenobacter gelipurpurascens Txg-1T (97.1 %). A number of phenotypic characteristics distinguished strain POB6T from related members of the genus Hymenobacter. On the basis of the evidence presented in this study, a novel species, Hymenobacter tenuis sp. nov., is proposed, with POB6T (=KCTC 52271T=JCM 31659T) as the type strain.