Open AccessNiabella pedocola sp. nov., isolated from soil
Author(s) -
Ram Hari Dahal,
Jaisoo Kim
Publication year - 2016
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.001099
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , phylogenetics , genus , microbiology and biotechnology , bacteria , bacteroidetes , botany , gene , genetics , anatomy
An aerobic, Gram-stain-negative, oxidase-positive and catalase-negative, non-motile, non-spore-forming, rod-shaped, orange-pigmented bacterium designated strain R384 T was isolated from soil. Flexirubin-type pigments were present. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain R384 T formed a lineage within the family Chitinophagaceae of the phylum Bacteroidetes that was distinct from various species of the genus Niabella, including 'Niabella thaonhiensis' NHI-24 (98.47 % sequence similarity), Niabellaaurantiaca DSM 17617 T (96.89 %), Niabelladrilacis E90 T (96.63 %), Niabellahirudinis E96 T (96.61 %), Niabellatibetensis 15-4 T (95.53 %), Niabellasoli DSM 19437 T (94.81 %), Niabellaginsengisoli GR10-1 T (94.19 %) and Niabellayanshanensis CCBAU 05354 T (93.67 %). The major isoprenoid quinone was menaquinone-7 (MK-7) and the major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) and iso-C17 : 0 3-OH. The DNA G+C content of strain R384 T was 44.7 mol %. DNA-DNA hybridization values between strain R384 T and other members of the genus Niabella ranged from 26 to 55 %. On the basis of phenotypic, genotypic and phylogenetic analysis, strain R384 T represents a novel species of the genus Niabella, for which the name Niabellapedocola sp. nov. is proposed. The type strain is R384 T (=KEMB 9005-329 T =KACC 18454 T =JCM 31011 T ).