Open AccessNonomuraea thermotolerans sp. nov., a thermotolerant actinomycete isolated from mushroom compost
Author(s) -
Hao Wu,
Bin Liu
Publication year - 2016
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.000809
Subject(s) - biology , 16s ribosomal rna , mycelium , diamino acid , microbiology and biotechnology , peptidoglycan , strain (injury) , ribosomal rna , bacteria , phylogenetic tree , mushroom , botany , spore , cell wall , gene , biochemistry , gene sequence , genetics , anatomy
A novel thermotolerant actinomycete, designated strain 3-33-9B T , was isolated from mushroom compost in Nanning, Guangxi province, China. The strain grew at 25-60 °C, pH 7.0-11.0 and with 0-2.0 % (w/v) NaCl. Strain 3-33-9B T formed extensively branched aerial mycelia and substrate mycelia. Spiral spore chains with one to three turns were composed of about 3-15 non-motile spores with a wrinkled surface. The organism contained meso -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. Whole-cell hydrolysates contained madurose, galactose, glucose and ribose. The cellular fatty acid profile mainly consisted of iso-C 16 : 0 and C 17 : 0 . The quinone system was predominantly composed of MK-9(H 4 ). The DNA G+C content of strain 3-33-9B T was 72.5 mol%. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus Nonomuraea . Moreover, 16S rRNA gene sequence analysis also showed that the organism belonged to the genus Nonomuraea ; in the 16S rRNA gene tree of the genus Nonomuraea , strain 3-33-9B T formed a distinct phyletic line and was most closely related to Nonomuraea fastidiosa NBRC 14680 T (97.3 % 16S rRNA gene sequence similarity). However, phenotypic characteristics of strain 3-33-9B T were significantly different from N. fastidiosa NBRC 14680 T , and DNA-DNA hybridization showed little relatedness (31.6-35.2 %) between the two strains. Based on the phenotypic and phylogenetic data, strain 3-33-9B T represents a novel species of the genus Nonomuraea , for which the name Nonomuraea thermotolerans sp. nov. is proposed. The type strain is 3-33-9B T ( = ATCC BAA-2629 T = CGMCC 4.7161 T ).