Actinokineospora guangxiensis sp. nov., isolated from soil

A novel actinomycete, designated strain GK-6 T , was isolated from a soil sample from Nanning, Guangxi province, PR China. The strain grew at 20–40 °C, pH 6.0–11.0 and with 0–7.0 % NaCl. It formed well-developed aerial and vegetative mycelia. The aerial mycelium was white and the vegetative mycelium was yellow. The long branching aerial mycelia yielded rod-shaped arthrospores, the spores had smooth surfaces and were non-motile. Strain Gk-6 T contained meso-diaminopimelic acid as the diagnostic diamino acid, the whole-cell sugars were galactose, glucose and arabinose. Major fatty acids were iso-C 16 : 0 , iso-C 15 : 0 and C 17 : 0 . MK-9(H 4 ) was the predominant menaquinone. The polar phospholipids were phosphatidylethanolamine, phosphatidylethanolamine-containing hydroxylated fatty acids, diphosphatidylglycerol, ninhydrin-positive glycophospholipid and an unknown phospholipid. The G+C content of the genomic DNA was 73.4 mol%. The 16S rRNA gene sequence analysis indicated that the organism was a member of the genus Actinokineospora and its closest relative among recognized species was Actinokineospora soli JCM 17695 T (97.7 % sequence similarity). But the phenotypic characteristics of strain Gk-6 T were significantly different from those of A. soli JCM 17695 T , and DNA–DNA hybridization showed low relatedness (22.6–28.3 %) between strain Gk-6 T and JCM 17695 T . On the basis of the phenotypic and phylogenetic data, strain Gk-6 T represents a novel species of the genus Actinokineospora , and the name Actinokineospora guangxiensis sp. nov. is proposed. The type strain is Gk-6 T ( = DSM 46779 T  = CGMCC 4.7154 T ).