Open AccessPaenibacillus yunnanensis sp. nov., isolated from Pu'er tea
Author(s) -
Lili Niu,
Tianyi Tang,
Zhongliang Ma,
Lei Song,
Kegui Zhang,
Yuanyuan Chen,
Hua Zhang,
Xing Hu,
Meng Zhao
Publication year - 2015
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.000496
Subject(s) - biology , 16s ribosomal rna , paenibacillus , microbiology and biotechnology , peptidoglycan , diamino acid , diaminopimelic acid , phylogenetic tree , strain (injury) , bacteria , ribosomal rna , botany , cell wall , gene sequence , gene , genetics , anatomy
A novel Gram-staining-positive, aerobic, endospore-forming, rod-shaped bacterial strain, YN2 T , was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain represented a novel species of the genus Paenibacillus . The strains most closely related to strain YN2 T were Paenibacillus vulneris JCM 18268 T and Paenibacillus rigui JCM 16352 T , with 16S rRNA similarities of 98.6 and 95.5 %, respectively. Chemotaxonomic data supported the affiliation of the new isolate to the genus Paenibacillus , including MK-7 as the major menaquinone, DNA G+C content of 51 mol%, cell-wall type A1γ ( meso -diaminopimelic acid as the diagnostic diamino acid) and anteiso-C 15 : 0 and iso-C 16 : 0 as the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and phospholipid. Strain YN2 T could be differentiated from recognized species of the genus Paenibacillus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, Paenibacillus yunnanensis sp. nov., is proposed, with strain YN2 T ( = CGMCC 1.12968 T = JCM 30953 T ) as the type strain.