Open AccessAestuariivita atlantica sp. nov., isolated from deep-sea sediment
Author(s) -
Guizhen Li,
Qiliang Lai,
Yaping Du,
Xiupian Liu,
Fei Sun,
Zongze Shao
Publication year - 2015
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.000406
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , oxidase test , phosphatidylglycerol , microbiology and biotechnology , genus , bacteria , genomic dna , botany , flavobacteriaceae , gene , bacteroidetes , phosphatidylcholine , biochemistry , phospholipid , genetics , anatomy , enzyme , membrane
A novel strain, 22II-S11-z3 T , was isolated from the deep-sea sediment of the Atlantic Ocean. The bacterium was aerobic, Gram-staining-negative, oxidase-positive and catalase-negative, oval- to rod-shaped, and non-motile. Growth was observed at salinities of 1–9 % NaCl and temperatures of 10–45 °C. The isolate could hydrolyse aesculin and Tweens 20, 40 and 80, but not gelatin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S11-z3 T belonged to the genus Aestuariivita , with highest sequence similarity to Aestuariivita boseongensis KCTC 42052 T (97.5 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain 22II-S11-z3 T and A. boseongensis KCTC 42052 T were 71.5 % and 20.0 ± 2.3 %, respectively. The G+C content of the chromosomal DNA was 65.5 mol%. The principal fatty acids (>5 %) were summed feature 8 (C 18 : 1 ω7 c /ω6 c ) (35.2 %), C 19 : 0 cyclo ω8 c (20.9 %), C 16 : 0 (11.8 %), 11-methyl C 18 : 1 ω7 c (11.4 %) and C 12 : 1 3-OH (9.4 %). The respiratory quinone was determined to be Q-10. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, nine unidentified phospholipids, one unidentified aminolipid and two unidentified lipids were present. The combined genotypic and phenotypic data show that strain 22II-S11-z3 T represents a novel species of the genus Aestuariivita , for which the name Aestuariivita atlantica sp. nov. is proposed, with the type strain 22II-S11-z3 T ( = KCTC 42276 T = MCCC 1A09432 T ).