Oceanobacillus limi sp. nov., a moderately halophilic bacterium from a salt lake

A Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, moderately halophilic bacterium, designated strain H9B T , was isolated from a mud sample of the hypersaline lake Aran-Bidgol in Iran. Cells of strain H9B T were motile and produced colonies with a yellowish-grey pigment. Growth occurred between 2.5 and 10 % (w/v) NaCl and the isolate grew optimally at 7.5 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of pH 6–10 and 25–45 °C, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H9B T is a member of the genus Oceanobacillus . The closest relative to this strain was Oceanobacillus profundus CL-MP28 T with 97.1 % 16S rRNA gene sequences similarity. The level of DNA–DNA relatedness between the novel isolate and this phylogenetically related species was 17 %. The major cellular fatty acids of the isolate were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 and iso-C 16 : 0 . The polar lipid pattern of strain H9B T consisted of phosphatidylglycerol, diphosphatidylglycerol, four phospholipids and an aminolipid. It contained MK-7 as the predominant menaquinone and meso -diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 37.1 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus Oceanobacillus , for which the name Oceanobacillus limi sp. nov. is proposed. The type strain of Oceanobacillus limi is strain H9B T ( = IBRC-M 10780 T  = KCTC 13823 T  = CECT 7997 T ).