Open AccessRapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay
Author(s) -
Richard D. Smith,
Jerilyn R. Izac,
Michael Ha,
Hyojik Yang,
J. Kristie Johnson,
Robert K. Ernst
Publication year - 2021
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000309
Subject(s) - colistin , escherichia coli , urine , mass spectrometry , ex vivo , microbiology and biotechnology , urinary system , matrix assisted laser desorption/ionization , antibiotics , mcr 1 , chemistry , chromatography , medicine , biology , enterobacteriaceae , biochemistry , in vitro , gene , desorption , organic chemistry , adsorption
Mobilized colistin resistance ( mcr ) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic used to rapidly identify an mcr-1 -positive Escherichia coli directly from a patient with a urinary tract infection without the need for ex vivo growth.