Open AccessInteraction sites of the Epstein–Barr virus Zta transcription factor with the host genome in epithelial cells
Author(s) -
Anja Godfrey,
Kay Osborn,
Alison J. Sinclair
Publication year - 2021
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000282
Subject(s) - lytic cycle , bzlf1 , biology , epstein–barr virus , chromatin immunoprecipitation , transcription factor , chromatin , gene , microbiology and biotechnology , cell cycle , dna replication , virus , virology , gene expression , herpesviridae , promoter , genetics , viral disease
Epstein–Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14 000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites.