Open AccessGenetic characterization of co-trimoxazole non-susceptible Streptococcus pneumoniae isolates from Indonesia
Author(s) -
Dodi Safari,
Hanifah Fajri Maharani Putri,
Arrayan Bimantari,
Wisiva Tofriska Paramaiswari,
Wisnu Tafroji,
Miftahuddin Majid Khoeri,
Korrie Salsabila
Publication year - 2021
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000271
Subject(s) - dhps , dihydrofolate reductase , dihydropteroate synthase , streptococcus pneumoniae , biology , microbiology and biotechnology , gene , sulfamethoxazole , trimethoprim , polymerase chain reaction , population , virology , genetics , pyrimethamine , medicine , antibiotics , immunology , plasmodium falciparum , environmental health , malaria
We investigated the genetic variation of folA and folP genes encoding dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR) enzymes amongst trimethoprim/sulfamethoxazole (co-trimoxazole) non-susceptibleStreptococcus pneumoniaeisolated from the Indonesian population. ArchivedS. pneumoniaeisolates were screened for the presence and analysis of folA and folP genes using the polymerase chain reaction sequencing method. We found that 80 % of co-trimoxazole non-susceptible isolates ( n =30/39) showed a 6 bp insertion in the sulphonamide-binding site of DHPS. The Asp-92-Ala and Ile-100-Leu substitutions were more common on DHFR (42 %; 22/53) followed by the Asp-92–Ala, Glu-94–Asp and Ile-100–Leu substitutions (32 %; 17/53). The combination of the Ile-100–Leu substitution at the DHFR region and the 6 bp insertion was the most dominant combination among isolates having both folA and folP genes.