Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies | Zendy

Inna Sekirov | Zendy; Martin Petric | Zendy; Erin Carruthers | Zendy; David S. Lawrence | Zendy; Tamara Pidduck | Zendy; Jesse Kustra | Zendy; Jonathan Laley | Zendy; Min-Kuang Lee | Zendy; Navdeep Chahil | Zendy; Annie Mak | Zendy; Paul N. Levett | Zendy; Emelissa J. Mendoza | Zendy; Heidi Wood | Zendy; Mike Drebot | Zendy; Mel Krajden | Zendy; Muhammad Morshed | Zendy

Open Access

Performance comparison of micro-neutralization assays based on surrogate SARS-CoV-2 and WT SARS-CoV-2 in assessing virus-neutralizing capacity of anti-SARS-CoV-2 antibodies

Author(s) -

Inna Sekirov,

Martin Petric,

Erin Carruthers,

David S. Lawrence,

Tamara Pidduck,

Jesse Kustra,

Jonathan Laley,

Min-Kuang Lee,

Navdeep Chahil,

Annie Mak,

Paul N. Levett,

Emelissa J. Mendoza,

Heidi Wood,

Mike Drebot,

Mel Krajden,

Muhammad Morshed

Publication year - 2021

Publication title -

access microbiology

Language(s) - English

Resource type - Journals

ISSN - 2516-8290

DOI - 10.1099/acmi.0.000257

Subject(s) - neutralization , virology , covid-19 , antibody , neutralizing antibody , coronavirus , virus , coronaviridae , biology , medicine , immunology , outbreak , infectious disease (medical specialty) , disease , pathology

We compared neutralization assays using either the wild-type severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus or surrogate neutralization markers, using characterized sera. We found the results of the neutralization assays 75 % concordant overall and 80 % concordant for samples with high antibody levels. This demonstrates that commercial surrogate SARS-CoV-2 assays offer the potential to assess anti-SARS-CoV-2 antibodies’ neutralizing capacity outside CL-3 laboratory containment.

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