Open AccessIn vitro growth-inhibitory effects of Portulaca oleracea L. formulation on intestinal pathogens
Author(s) -
Sae Okuda,
Takeaki Wajima,
Tetsuya Yamada,
Hidemasa Nakaminami,
Hideaki Ikoshi,
Norihisa Noguchi
Publication year - 2021
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000208
Subject(s) - portulaca , shigella dysenteriae , vibrio cholerae , microbiology and biotechnology , shigella , biology , dysentery , cholera , plesiomonas shigelloides , aeromonas , cholera toxin , salmonella , bacteria , escherichia coli , biochemistry , botany , genetics , gene
Introduction Empirical evidence suggests that Portulaca oleracea L. treats enteric infections, including dysentery, cholera, and acute infectious gastroenteritis. Aim The aim of this study is to clarify the growth-inhibitory effects of Portulaca oleracea L. extract against 56 strains of intestinal pathogens. Methodology ‘Gogyo-so-cha (GSC)’ was used as the P. oleracea L. formulation. A growth curve analysis was used to measure the growth-inhibitory effects of GSC, and Shiga toxin induction was measured using the latex agglutination test. Results GSC demonstrated strong bactericidal effects againstShigella dysenteriaeandVibrio choleraestrains from various isolates. GSC demonstrated weak or no bactericidal effects against intestinal commensal bacteria, includingEnterococcusspp. andEscherichia coli . GSC did not induceShigellatoxins. Conclusion GSC significantly inhibited the growth of intestinal pathogens, includingS. dysenteriaeandV. cholerae , without adversely affecting the intestinal flora, supporting the usage of GSC in traditional Chinese medicine. Taken together, GSC would be of immense value in the developing world, where diarrhoeal infectious diseases continue to pose a major health risk.