Open AccessA case of canine cutaneous pythiosis in Thailand
Author(s) -
Ariya Chindamporn,
Patcharee Kammarnjessadakul,
Sawang Kesdangsakonwut,
Wijit Banlunara
Publication year - 2020
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000109
Subject(s) - biology , internal transcribed spacer , polymerase chain reaction , pathology , clade , gene , phylogenetic tree , medicine , genetics
Pythium insidiosum causes pythiosis in humans and animals in tropical and subtropical climates. The clinical manifestations in humans are mostly systemic, vascular or ocular forms, in contrast to animals, which are cutaneous, subcutaneous and gastrointestinal forms. The highest incidence of human cases is reported in Thailand, however, no canine pythiosis has been documented yet. Case presentation A female, mixed-breed, stray dog showed severe extensive ulcerative haemorrhagic dermatitis at the perineum involving the anus and tail. On cytology, there were sparse branching septate fungal hyphae. The tissue samples were subjected to polymerase chain reaction and gene sequencing for fungal identification. Conclusion The results of the internal transcribed spacer 1 and 2 (ITS1 and ITS2) gene had 99 % homology to Pythium insidiosum (accession no. FJ17396) and the COX2 gene (accession no. GQ451572). The phylogenetic tree of both genes was classified in clade A TH. This is the first fully documented diagnosis of canine cutaneous pythiosis in Thailand.