Molecular characterization of the first reported Aichivirus A in Australia | Zendy

Judy A Northill | Zendy; Russell J. Simmons | Zendy; Doris Genge | Zendy; Frederick Moore | Zendy

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Molecular characterization of the first reported Aichivirus A in Australia

Author(s) -

Judy A Northill,

Russell J. Simmons,

Doris Genge,

Frederick Moore

Publication year - 2020

Publication title -

access microbiology

Language(s) - English

Resource type - Journals

ISSN - 2516-8290

DOI - 10.1099/acmi.0.000099

Subject(s) - norovirus , genotyping , genotype , outbreak , polymerase chain reaction , biology , virology , reverse transcription polymerase chain reaction , genome , microbiology and biotechnology , genetics , gene , messenger rna

A novel real-time reverse transcription polymerase chain reaction (RT-rPCR) assay was developed to detect Aichivirus A (AiV-A) based on four complete genomes. The assay successfully detected AiV-A in a sample from a patient with acute gastroenteritis in January 2008. Screening of 756 samples submitted for norovirus testing during May 2008 detected a further 23 AiV-A-positive samples from 18 individual patients. Genotyping using novel primers targeting the 3C–3D junction region identified AiV-A genotype B. Further sequencing of the VP1 region supported the 3C–3D result. All three assays proved useful to support foodborne outbreak investigations. This is the first report of AiV-A detection in Australia.

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