Open AccessPhenotypic characterization of Neisseria meningitidis strains isolated from invasive meningococcal disease in Brazil from 2002 to 2017
Author(s) -
Maria Cecília Outeiro Gorla,
Angela Pires Brandão,
Juliana Maíra Watanabe Pinhata,
Camile de Moraes,
Geraldo Pereira,
Ana Paula Silva de Lemos
Publication year - 2020
Publication title -
access microbiology
Language(s) - English
Resource type - Journals
ISSN - 2516-8290
DOI - 10.1099/acmi.0.000079
Subject(s) - neisseria meningitidis , meningococcal disease , serotype , neisseriaceae , case fatality rate , microbiology and biotechnology , epidemiology , biology , virology , medicine , antibiotics , bacteria , genetics
Invasive meningococcal disease (IMD) has a high rate of fatality and may cause severe clinical sequelae. Over the years, the epidemiology of IMD has changed significantly in various regions of the world, and laboratory surveillance of this disease is important for mapping epidemiologic changes. Aim To perform phenotypic characterization ofNeisseria meningitidisstrains isolated from invasive disease in Brazil from 2002 to 2017, as a complementation of the data obtained in the period of 1990–2001. Methodology In total, 8,689 isolates sent to Adolfo Lutz Institute confirmed asN. meningitidisby conventional methods were serogrouped by slide agglutination against MenA, MenB, MenC, MenE, MenW, MenX, MenY and MenZ; serotyped and serosubtyped by a whole-cell dot-blotting assay with monoclonal antibodies. Results The isolates were sent from all regions of Brazil, and the southeast region was responsible for the largest number of isolates (57.2 %). Overall, the total sample ( n =8,689) was represented by serogroups C ( n =4,729; 54.4 %), B ( n =3,313; 38.1 %), W ( n =423; 4.9 %), Y ( n =203; 2.3 %), X ( n =5; 0.1 %) and others ( n =16; 0.2 %). A shift in the prevalence of serogroups was observed in 2006, when serogroup C became the most prevalent (65.5 %), surpassing the serogroup B (21.9 %). The main isolated phenotypes were C:23:P1.14–6; B:4,7:P1.19,15; W:2a:P1.5 and W:2a:P1.5,2. Conclusion The data show an important change in the distribution of meningococcal serogroups, serotypes and subtypes occurring during 2002–2017. A continuous laboratory-based surveillance provides robust information to implement appropriate strategies to IMD control.