Open AccessThe production and characterisation of rabbit antiserum and murine monoclonal antibodies to Haemophilus ducreyi
Author(s) -
Geeta Y. Finn,
Q. N. Karim,
C. S. F. Easmon
Publication year - 1990
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-31-3-219
Subject(s) - haemophilus ducreyi , antiserum , monoclonal antibody , isotype , immunofluorescence , bordetella pertussis , microbiology and biotechnology , antibody , biology , hybridoma technology , western blot , haemophilus , virology , bacterial outer membrane , pasteurellaceae , haemophilus influenzae , bacteria , escherichia coli , immunology , antibiotics , biochemistry , gene , genetics
Rabbit antiserum and murine monoclonal antibodies were raised against a strain of Haemophilus ducreyi. The antiserum gave high immunofluorescence titres and strong dot blot reactions with all H. ducreyi strains tested and the only cross reaction was with Bordetella pertussis. Three monoclonal antibodies, all of isotype IgG2a, also gave high immunofluorescence titres with H. ducreyi but did not cross react with any other species tested. Immunoblotting showed the monoclonal antibodies to react with a single polypeptide band of mol. wt 29,000 in the outer-membrane fraction of H. ducreyi. These antibodies have potential for use as diagnostic reagents and for investigating the pathogenicity of H. ducreyi.