Open AccessGrowth of Yersinia Pseudotuberculosis in Mouse Spleen Despite Loss of a Virulence Plasmid of Mol. WT 47 x 106
Author(s) -
Michel Simonet,
D Mazigh,
Patrick Berche
Publication year - 1984
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-18-3-371
Subject(s) - virulence , plasmid , yersinia pseudotuberculosis , microbiology and biotechnology , biology , strain (injury) , spleen , bacteria , enterobacteriaceae , in vivo , yersinia , escherichia coli , gene , genetics , immunology , anatomy
A highly virulent strain of Yersinia pseudotuberculosis (LD50 c. 10(2) bacteria/mouse) harboured two plasmids with mol. wt of 47 X 10(6) and 61 X 10(6). The role of these plasmids in virulence was studied in mice with derived strains cured of plasmids. It was confirmed that the plasmid of mol. wt 47 X 10(6) played a major function in virulence. This was shown both by the increase of the LD50 and the lower rate of multiplication in the spleen obtained with strains cured of the plasmid of mol. wt 47 X 10(6). The plasmid of mol. wt 61 X 10(6) did not play any role in virulence. This work also demonstrates that the strain cured of the plasmid of mol. wt 47 X 10(6) and the plasmid-free strain were able to multiply in the spleens of infected mice during a 7-day period. This suggests that virulence factors not associated with plasmids are also responsible for the bacterial growth in tissues in vivo.