Open AccessReactions of extracts of Reiter treponeme with syphilitic and non-syphilitic human sera in the single radial haemolysis technique
Author(s) -
A. A. Al-Qudah,
Ann Mostratos
Publication year - 1984
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-17-3-273
Subject(s) - syphilis , haemolysis , complement fixation test , syphilis serodiagnosis , antibody , antigen , medicine , immunology , chemistry , virology , serology , human immunodeficiency virus (hiv)
A single radial haemolysis (SRH) technique, using Reiter protein or Reiter lysate as the coating antigen, was investigated. Results obtained with syphilitic and presumed non-syphilitic human sera were compared with results obtained in the absorbed fluorescent treponemal antibody test (FTA-ABS), the Reiter protein complement fixation test ( RPCFT ), the Venereal Diseases Research Laboratory Slide test (VDRL) and the Cardiolipin Wasserman reaction ( CWR ). The SRH reaction, with either Reiter antigen, was more sensitive than any of the screening tests ( RPCFT , VDRL and CWR ) for detecting positive syphilitic antibodies. Although the SRH test used almost the same materials as the RPCFT , it was appreciably more sensitive for the detection of the group-specific antibodies in syphilitic human serum.