Open AccessA Biotyping Scheme for the Subspecific Discrimination of Escherichia Coli
Author(s) -
Pamela B. Crichton,
D. C. Old
Publication year - 1982
Publication title -
journal of medical microbiology/journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/00222615-15-2-233
Subject(s) - biology , escherichia coli , sorbose , rhamnose , microbiology and biotechnology , raffinose , strain (injury) , primary (astronomy) , fermentation , bacteria , actinomycetales , genetics , streptomyces , biochemistry , galactose , fructose , gene , physics , anatomy , astronomy , sucrose
A two-tier system for biotyping Escherichia coli gave a fine and reliable differentiation of strains and is capable of future extension by the addition of new types and new tests. Strains were allocated to a primary biotype (1-16) by their reactions in four primary tests in culture media containing raffinose, sorbose, ornithine or dulcitol. Subtypes were distinguished within the primary biotypes by reactions in six secondary tests for rhamnose fermentation, lysine decarboxylation, aesculin hydrolysis, motility, type-1 fimbriation and prototrophy. Full biotypes were designated by letters indicating subtype reactions appended to primary type numbers. A series of 599 strains (1242 cultures) of E. coli from diverse sources was classified into 16 primary biotypes and into 213 full biotypes. The biotype characters of a strain were generally stable during its spread in the natural environment and in non-selective media used for storage.