Open AccessRole of Phospholipid Head Groups in Ethanol Tolerance of Saccharomyces cerevisiae
Author(s) -
Prashant Mishra,
Rajendra Prasad
Publication year - 1988
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-134-12-3205
Subject(s) - efflux , saccharomyces cerevisiae , phospholipid , phosphatidylserine , ethanol , yeast , biochemistry , chemistry , incubation , membrane , alanine , biology , amino acid
Pre-incubation of cells of Saccharomyces cerevisiae with 2 M-ethanol led to decreased rates of L-alanine uptake, H+ efflux and fermentation rate. However, these responses were modified in yeast cells with altered phospholipid composition. Using L-alanine transport and H+ efflux as indices of ethanol tolerance, it was observed that cells enriched with phosphatidylserine had greater tolerance to ethanol. This resulted from altered charge of membrane phospholipids rather than changes in membrane fluidity. It is suggested that the anion:zwitterion ratio of phospholipids may be one of the important determinants of ethanol tolerance in S. cerevisiae.