Open AccessTransduction Complicates the Detection of Conjugative Ability in Lysogenic Salmonella Strains
Author(s) -
Smith Hw,
Lovell Ma
Publication year - 1985
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-131-8-2087
Subject(s) - lysogenic cycle , salmonella , microbiology and biotechnology , plasmid , transduction (biophysics) , streptomycin , biology , strain (injury) , bacteriophage , bacteria , virology , genetics , escherichia coli , gene , antibiotics , biochemistry , anatomy
When lysogenic salmonella strains were examined for conjugative ability in tri-parental crosses, false positive results were sometimes obtained because phage carried by the lysogenic strains multiplied on the intermediate salmonella recipient strain and then transduced its streptomycin/sulphonamide resistance plasmid to the final salmonella recipient strain. Back transfer of the plasmid to the lysogenic strains was also detected.