Open AccessTransfection of Corynebacterium lilium Protoplasts
Author(s) -
Patrice Yeh,
J Oreglia,
Sicard Am
Publication year - 1985
Publication title -
microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-131-12-3179
Subject(s) - protoplast , lilium , corynebacterium , microbiology and biotechnology , transfection , biology , botany , bacteria , genetics , gene
A protoplast transfection system has been developed for a lysine-producing bacterium, Corynebacterium lilium, using the DNA of phage CL31. Phage CL31 is lytic and specific to C. lilium and has a genome of approximately 48 kb. The transfection procedure involves a polyethylene-glycol-mediated introduction of the DNA into lysozyme-treated cells and has a maximum efficiency of 3 X 10(4) transfectants per microgram DNA.