Open AccessPlasmid Transformation of Bacillus sphaericus 1593
Author(s) -
Karen Orzech McDonald,
William F. Burke
Publication year - 1984
Publication title -
microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-130-1-203
Subject(s) - bacillus sphaericus , plasmid , transformation (genetics) , protoplast , microbiology and biotechnology , biology , strain (injury) , bacillaceae , bacteria , bacillales , genetics , gene , bacillus subtilis , anatomy
Plasmids pUB110 and pBC16 were introduced by protoplast transformation into the entomocidal bacterium Bacillus sphaericus 1593. Transformants expressed the antibiotic resistance determinants present on the plasmid and exhibited sporulation frequencies and larvicidal toxicities which were equivalent to those characteristic of the parent strain. These transformations constitute the first report of genetic transfer in B. sphaericus.