Open AccessImmunochemistry of the Cell-Surface Carbohydrate Antigens of Clostridium difficile
Author(s) -
Ian R. Poxton,
T.D.I. Cartmill
Publication year - 1982
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-128-6-1365
Subject(s) - teichoic acid , galactosamine , immunochemistry , mannose , antigen , antiserum , glucosamine , biochemistry , clostridium , bacteria , chromatography , cell wall , carbohydrate , chemistry , microbiology and biotechnology , biology , sepharose , antibody , peptidoglycan , immunology , enzyme , genetics
Two carbohydrate cell-surface antigens were extracted from Clostridium difficile. One was extracted from pure cell walls by NaOH and contained glucose, mannose, galactosamine and phosphate in the approximate molar proportions of 2:0.65:1:0.63. The other antigen was extracted with phenol from the disrupted contents of whole cells and purified by chromatography on Sepharose 6B and an immunoabsorbent column; it contained glucose, glucosamine, phosphate and fatty acid in the approximate molar proportions of 2:1:1.6:0.04. Both antigens showed partial immunological identity and both cross-reacted with Clostridium sordellii antiserum. They are analogues of the wall and membrane teichoic acids of other Gram-positive bacteria.