Open AccessA Mutant Inducible for Galactitol Utilization in Escherichia coli K12
Author(s) -
Christos Delidakis,
M. C. JonesMortimer,
H. L. Kornberg
Publication year - 1982
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.352
H-Index - 35
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-128-3-601
Subject(s) - galactitol , escherichia coli , pep group translocation , mutant , biochemistry , biology , enzyme , enterobacteriaceae , phosphoenolpyruvate carboxykinase , salmonella , microbiology and biotechnology , chemistry , bacteria , gene , genetics , galactose
Galactitol-positive strains of Escherichia coli K12 are inhibited by the galactitol analogues L-fucitol and 2-deoxy-D-galactitol, but not by D-fucitol; Salmonella typhimurium LT2 is not inhibited by these compounds. Most mutants selected as resistant to either toxic compound are unable to utilize galactitol as carbon source, but a relatively rare class is inducible for the Enzyme II of the galactitol:phosphoenolpyruvate phosphotransferase system, the product of which is D-galactitol 6-phosphate. The lesion in one such mutant maps near metG at about min 45 on the E. coli genome.