Open AccessProduction, Purification and Chemical Properties of an Antistaphylococcal Agent Produced by Staphylococcus epidermidis
Author(s) -
HansGeorg Sahl,
H. Brandis
Publication year - 1981
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-127-2-377
Subject(s) - bacteriocin , pronase , isoelectric point , sephadex , lysostaphin , chemistry , chromatography , ninhydrin , trypsin , staphylococcus epidermidis , chymotrypsin , micrococcus luteus , hydrolysis , biochemistry , staphylococcus aureus , amino acid , bacteria , biology , enzyme , antimicrobial , escherichia coli , organic chemistry , gene , genetics
An antibacterial agent produced by Staphylococcus epidermidis strain 5 was isolated from culture supernatants; its spectrum of activity is restricted to staphylococci and micrococci. The bacteriocin-like substance was purified to homogeneity by column chromatography on Servachrome XAD-2, CM-Sephadex C-25 and Sephadex G-50. Chemical analysis showed it to be a peptide with a molecular weight of about 6000 and an isoelectric point of about 10.5. Purified material was rapidly inactivated by trypsin, chymotrypsin and pronase and it was relatively heat-stable. After acidic hydrolysis 10 amino acid residues and two unidentified ninhydrin-positive substances were detected. Production of the bacteriocin-like substance was not inducible with mitomycin C.