Open AccessSome Properties of the Clostridium butyricum Group -Lactamase
Author(s) -
Michel Magot
Publication year - 1981
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-127-1-113
Subject(s) - clostridium butyricum , cefoxitin , benzylpenicillin , carbenicillin , cephalosporin , microbiology and biotechnology , cloxacillin , chemistry , penicillin , ampicillin , enzyme , isoelectric point , biology , biochemistry , antibiotics , bacteria , staphylococcus aureus , fermentation , genetics
Clostridium strain CB269, belonging to the butyricum group, produces a beta-lactamase. The substrate profile of this enzyme, as characterized by the microiodometric method, was that of a penicillinase: activity was slightly higher on ampicillin than on benzylpenicillin, while carbenicillin and methicillin were hydrolysed at lower rates. There was poor activity with cephalosporins. The enzyme was excreted during growth, and was inducible to a limited extent by cloxacillin or cefoxitin. The isoelectric point of the enzyme was approximately 4.1.