Open AccessA Simple Method for the Isolation of Flagellar Shape Mutants in Salmonella
Author(s) -
Hideo Fujita,
Shigeru Yamaguchi,
Toshifumi Taira,
Tetsuo Iino
Publication year - 1981
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-125-1-213
Subject(s) - mutant , flagellum , salmonella , microbiology and biotechnology , isolation (microbiology) , biology , serial dilution , bacteria , genetics , gene , medicine , alternative medicine , pathology
During prolonged static cultivation of liquid cultures of Salmonella strains with normal flagella, flagellar shape mutants occurred spontaneously and their proportion in the cultures increased so that they were easily isolated by spreading dilutions of the cultures on semisolid medium. By this method various types of flagellar shape mutants, including curly, straight and polymorphous mutants, were obtained.