Open AccessTaxonomic Significance of Yeast Sphaeroplast Release after Enzymic Treatment of Intact Cells
Author(s) -
J.-M. Bastide,
E. Hadibi,
M. Bastide
Publication year - 1979
Publication title -
journal of general microbiology/journal of general microbiology
Language(s) - English
Resource type - Journals
eISSN - 2059-9323
pISSN - 0022-1287
DOI - 10.1099/00221287-113-1-147
Subject(s) - yeast , fungi imperfecti , glucanase , trichoderma viride , biology , trichoderma , enzyme , microbiology and biotechnology , basidiomycota , botany , biochemistry
Treatment of whole yeast cells with a mixture of a reducing agent and 1,3-beta-glucanase isolated from Basidiomycete QM806 led to the production of sphaeroplasts from ascomycetes, from some fungi imperfecti, but not from basidiomycetes. Association of 1,3-beta-glucanase with a second enzyme, 1,4-alpha-glucanase, from Trichoderma viride, was required for sphaeroplast release from some, but not all, basidiomycetes and fungi imperfecti. The ability of yeast cells to liberate sphaeroplasts following appropriate enzymic treatment is proposed as a taxonomic criterion for differentiating basidiomycetous from ascomycetous yeasts and for classifying fungi imperfecti yeasts.