Open AccessDemonstration of bovine CD8+ T-cell responses to foot-and-mouth disease virus.
Author(s) -
A. Childerstone,
L. Cedillo-Baron,
Mildred Foster-Cuevas,
R. M. E. Parkhouse
Publication year - 1999
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-80-3-663
Subject(s) - biology , foot and mouth disease virus , cytotoxic t cell , virology , cd8 , virus , foot and mouth disease , immunity , immunology , disease , antigen , major histocompatibility complex , immune system , cellular immunity , in vitro , medicine , genetics , pathology
The aim of this study was to investigate the importance of cellular immunity in foot-and-mouth disease in cattle, in particular to determine whether a CD8+ T-cell response could be detected, as these cells may play a role in both immunity and virus persistence. As attempts to characterize classical cytotoxic T cells had yielded non-reproducible results, largely due to high backgrounds in control cultures, a proliferation assay was developed that was demonstrated to detect antigen-specific, MHC class I-restricted bovine CD8+ cells responding to foot-and-mouth disease virus (FMDV). Proliferative CD8+ T-cell responses were detected consistently from 10 to 14 days following infection with FMDV and typically lasted 3-4 weeks. The role of CD8+ T cells in control of the disease, in particular their relevance for the establishment of persistence, may now be investigated.