Open AccessComplete DNA sequence of canine adenovirus type 1.
Author(s) -
Mark Morrison,
David Onions,
Lesley Nicolson
Publication year - 1997
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-78-4-873
Subject(s) - orfs , biology , virology , dna , dna sequencing , genetics , genome , adenovirus genome , sequence (biology) , sequence analysis , nucleic acid sequence , mastadenovirus , direct repeat , microbiology and biotechnology , adenoviridae , recombinant dna , gene , open reading frame , peptide sequence
The complete DNA sequence of a field strain of canine adenovirus type 1 was determined by sequencing random fragments of viral DNA cloned into pBluescript. The virus has a genome of 30536 bp flanked by two identical 161 bp inverted terminal repeats. Thirty ORFs have been identified, based on genomic location or sequence identity with published adenoviruses. These are arranged into similar discrete regions found in the human adenoviruses. ORFs in the late region show greatest identity with published human adenovirus sequences, whereas the E3 and E4 ORFs show little or none.