Open AccessIsolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae
Author(s) -
Olli Vapalahti,
Åke Lundkvist,
Sami Kukkonen,
Ying Cheng,
Mari Gilljam,
Mari Kanerva,
Tytti Manni,
M Pejčoch,
Jukka Niemimaa,
Asko Kaikusalo,
Heikki Henttonen,
Antti Vaheri,
Alexander Plyusnin
Publication year - 1996
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-77-12-3063
Subject(s) - biology , virology , hantavirus , bunyaviridae , serotype , microtus , serology , puumala virus , hantaan virus , virus , antibody , immunology , zoology
A Vero E6 cell culture isolate of Tula virus (TUL), a hantavirus first detected in European common voles (Microtus arvalis and M. rossiaemeridionalis) by RT-PCR was obtained after initial passaging of TUL-infected vole lung samples in laboratory-colonized M. arvalis. TUL was defined as a classical serotype by a cross-focus-reduction neutralization test (FRNT) and was also shown to be distinct from other hantaviruses by haemagglutination inhibition assay. The sequences of S, M and partial L genome segments of the isolate were determined: the S segment was 99.9% identical to the original rodent-derived sequence. Serological evidence for a previous TUL infection was obtained from the serum of a blood donor living near a TUL focus in Moravia, Czech Republic, showing at least a 16-fold higher FRNT titre to TUL as compared to Puumala or other hantaviruses.