Open AccessCharacterization of Vibrio cholerae EITor typing phage D10
Author(s) -
Bimal K. Chakrabarti,
Kausik Si,
Dhrubajyoti Chattopadhyay
Publication year - 1996
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-77-11-2881
Subject(s) - vibrio cholerae , biology , novobiocin , dna , microbiology and biotechnology , virology , genetics , bacteria , antibiotics
The Vibrio cholerae EITor typing phage D10 was characterized. The adsorption kinetics of the phage on V. cholerae MAK757 strain were biphasic in nature. Intracellular growth was characterized by an eclipse period, latent period and burst size which were 20 min, 25 min and 80 particles per cell respectively. The phage yield was dependent on the concentration and time of addition of DNA synthesis inhibitors such as nalidixic acid and novobiocin, and RNA synthesis inhibitors such as rifampicin. The 32+/-0.2 kb linear double-stranded DNA molecule has unique termini. A restriction map of the phage DNA was constructed with the enzymes BamHI, HindIII and PstI.