Open AccessProtection against cucumber mosaic virus (CMV) strains O and Y and chrysanthemum mild mottle virus in transgenic tobacco plants expressing CMV-O coat protein
Author(s) -
Mitsutoshi Nakajima,
Tetsuo Hayakawa,
Ikuo Nakamura,
Masahiko Suzuki
Publication year - 1993
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-74-2-319
Subject(s) - cucumovirus , biology , cucumber mosaic virus , virology , coat protein , virus , tobacco mosaic virus , strain (injury) , serology , tobamovirus , genetically modified crops , mottle , plant virus , transgene , rna , antibody , gene , genetics , anatomy
Transgenic tobacco expressing the coat protein (CP) of cucumber mosaic virus strain O (CMV-O) showed a significant level of protection against CMV strains O and Y. When inoculum concentrations were increased, the transformants showed a stronger level of protection against CMV-O than against CMV-Y. The substitution of an amino acid residue between CMV-O and CMV-Y, which is presumed to change the conformation of CP, may reflect the difference in susceptibility to these viruses. Furthermore the transgenic tobacco plants showed a significant level of protection against chrysanthemum mild mottle virus, a member of the cucumovirus group but with no serological relationship to CMV.