Open AccessComplete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
Author(s) -
Bertha Cecilia Ramírez,
Iván Lozano,
Luis-Miguel Constantino,
AnneLise Haenni,
Lee A. Calvert
Publication year - 1993
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-74-11-2463
Subject(s) - orfs , biology , nucleic acid sequence , open reading frame , genetics , intergenic region , coding region , virology , nucleotide , complementary dna , potyvirus , plant virus , virus , gene , peptide sequence , genome
The complete sequence of rice hoja blanca virus (RHBV) RNA4 has been determined, based on the sequence of the corresponding cDNA clones. RNA4 consists of 1991 nucleotides with two open reading frames (ORFs). One putative ORF is located in the 5'-proximal region of the viral RNA4; it encodes a protein of predicted M(r) 20076 which corresponds to the major non-structural protein that accumulates in RHBV-infected rice plants, and which bears limited sequence identity with the helper component of tobacco vein mottling potyvirus. The other ORF is located in the 5'-proximal region of the viral complementary RNA4 and encodes a protein of predicted M(r) 32,469. Between the two ORFs is an intergenic region of 524 nucleotides, part of which can theoretically adopt a stable stem-loop structure; the 5' and 3' ends can potentially base-pair over 16 nucleotides, producing a pan-handle configuration. These characteristics are in favour of an ambisense coding strategy for RHBV RNA4.