Open AccessSimultaneous detection and typing of genital human papillomavirus DNA using the polymerase chain reaction
Author(s) -
Yukako Fujinaga,
Masamitu Shimada,
Kazuhide Okazawa,
Michio Fukushima,
Ikunoshin Kato,
Kei Fujinaga
Publication year - 1991
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-72-5-1039
Subject(s) - polymerase chain reaction , primer (cosmetics) , virology , biology , typing , human papillomavirus , dna , microbiology and biotechnology , restriction enzyme , sex organ , papillomaviridae , hpv infection , cervical cancer , gene , cancer , medicine , genetics , chemistry , organic chemistry
A simple method has been developed for detecting a broad range of genital human papillomavirus (HPV) types using the polymerase chain reaction (PCR). We utilized two consensus sequence primer pairs within the E6 and E7 open reading frames to amplify HPV DNA; malignant HPV DNA (from HPV-16, -18, -31, -33, -52b and -58) was amplified using the pU-1M/pU-2R primer pair whereas benign HPV DNA (from HPV-6 and -11) was amplified using the pU-31B/pU-2R primer pair. Identification of the amplification product was confirmed by restriction enzyme digestion. In this study, a pU-1M/pU-2R-mediated PCR was successfully applied to 39 cervical carcinoma specimens; HPV-16 was detected in 19 cases, HPV-18 in five cases, HPV-31 in two cases, HPV-33 in two cases, HPV-52b in one case, HPV-58 in three cases, and an unknown type(s) was detected in four cases. Overall, the prevalence of HPV was 84.6%. The results indicate that this detection system is useful for the detection of HPVs not only of known types but also of new types.