Open AccessConversion of a Single-stranded Simian Virus 40 (SV40)-based Shuttle Vector to its Double-stranded Form does not Require the SV40 T antigen in Monkey Cells
Author(s) -
Catherine Madzak,
Alain Sarasin
Publication year - 1991
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-72-12-3091
Subject(s) - simian , biology , virology , shuttle vector , transfection , dna , vector (molecular biology) , virus , sv40 large t antigen , antigen , origin of replication , microbiology and biotechnology , dna replication , cell culture , recombinant dna , gene , genetics
We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.