Open AccessIdentification and Characterization of the Virion Protein Products of Herpes Simplex Virus Type 1 Gene UL47
Author(s) -
Gordon W. McLean,
Frazer J. Rixon,
Nina Langeland,
Lars Haarr,
Howard S. Marsden
Publication year - 1990
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-71-12-2953
Subject(s) - biology , herpes simplex virus , virology , identification (biology) , gene , virus , microbiology and biotechnology , genetics , botany
We have identified two encoded proteins with an antiserum raised against a synthetic oligopeptide corresponding to amino acids 671 to 684 of the predicted protein product of gene UL47 of herpes simplex virus type 1 (HSV-1). They have apparent Mrs of 82,000 and 81,000 and are both major virion components located in the tegument. The 82/81K proteins were first detected in infected cells in minor amounts 6 h after infection at 37 degrees C but were later (from 10 h until 24 h after infection) present in large amounts. UL47 regulation was investigated using phosphonoacetic acid (PAA), an inhibitor of DNA synthesis: the amounts of the 82/81K protein synthesized were compared with those of 65KDBP, an early gene product, and 21K/22K, a true late gene product. The data showed that UL47 is regulated as a true late gene.