Open AccessA Herpes Simplex Virus Type 2 Variant in which a Deletion across the L-S Junction Is Replaced by Single or Multiple Reiterations of Extraneous DNA
Author(s) -
J Harland,
Stuart A. Brown
Publication year - 1989
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-70-8-2121
Subject(s) - biology , herpes simplex virus , genome , dna , population , strain (injury) , genetics , microbiology and biotechnology , virology , virus , gene , demography , anatomy , sociology
We have isolated and characterized a novel variant of herpes simplex virus type 2 (HSV-2) strain HG52 which has a deletion of 13.5 kb across the L-S junction of the genome, resulting in all of the IRL region, half of the IRS region and the intervening L-S junction 'a' sequence(s) being removed. The deleted DNA has been replaced by single or multiple (up to 14) reiterations of a DNA sequence approximately 1 kb in length. Individual genomes within the population range in size from approximately 12 kb smaller than unit length to unit length or marginally larger. The L component of the genome is fixed in the prototype orientation while the S component inverts inefficiently. The variant is viable in tissue culture, is not temperature-sensitive, demonstrates impaired single-cycle growth characteristics and, apart from altered mobility of a single species (29.5K), its polypeptide profile in infected cells appears normal. Southern blot analysis has failed to identify the inserted sequences as being derived from HSV-2.