Open AccessMolecular Cloning of a Potato Virus Y Genome: Nucleotide Sequence Homology in Non-coding Regions of Potyviruses
Author(s) -
Tom Turpen
Publication year - 1989
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/0022-1317-70-8-1951
Subject(s) - biology , complementary dna , genome , virology , genetics , potato virus y , nucleic acid sequence , cloning (programming) , coding region , homology (biology) , virus , microbiology and biotechnology , plant virus , dna , gene , computer science , programming language
An aphid-transmissible field isolate of potato virus Y, strain MM, was purified from a pepper host and cloned. All but 18 terminal nucleotides of the 9.7 kb genome are apparently contained in two overlapping cDNA clones. The library of cDNA clones is likely to be representative of the viral RNA population present in infected plants because restriction endonuclease maps derived from cloned and uncloned cDNA are colinear, each region of the genome is represented by several independent clones, and nucleic acid sequencing of 5' and 3'-terminal regions revealed small AU-rich non-coding domains with blocks of nucleotide sequence homology between this strain of PVY and three other potyviruses. The efficient application of cDNA cloning techniques to a large positive-stranded RNA virus is described.